TY - JOUR
T1 - Biochemistry of bovine lens proteins II. The γ-crystallins of adult bovine, calf and embryonic lenses
AU - Papaconstantinou, John
N1 - Funding Information:
This research project was supported by grant No. CRMS-x85 from the National Foundation and grant No. NB-o4455-o2 from the U.S.P.H.S.
PY - 1965/8/24
Y1 - 1965/8/24
N2 - An analysis of the soluble proteins from the adult and calf bovine lens epithelial cells by DEAE-cellulose fractionation has shown that these cells do not contain the γ-crystallins, but do contain the normal complement of α-andβ-crystallins. Significant quantities of γ-crystallins could be found, however, in the fiber cells of adult, calf and embryonic lenses. It was concluded from these experiments that the initiation of γ-crystallin synthesis is specifically associated with the differentiation of lens epithelial cells to lens fiber cells. Resolution of teh γ-crystallin of adult cortex, adult nucleus and embryonic lens was achieved by fractionation on DEAE-cellulose columns. Electrophoretic analysis of the purified γ-crystallin showed that those of the embryo and adult nucleus have similar mobilities whereas the mobility of adult cortex γ-crystallins differed from the others. It appears, therefore that the γ-crystallins formed in fiber cell differentiation in an embryonic lens are electrophoretically distinct from those formed during fiber differentiation in an adult lens.
AB - An analysis of the soluble proteins from the adult and calf bovine lens epithelial cells by DEAE-cellulose fractionation has shown that these cells do not contain the γ-crystallins, but do contain the normal complement of α-andβ-crystallins. Significant quantities of γ-crystallins could be found, however, in the fiber cells of adult, calf and embryonic lenses. It was concluded from these experiments that the initiation of γ-crystallin synthesis is specifically associated with the differentiation of lens epithelial cells to lens fiber cells. Resolution of teh γ-crystallin of adult cortex, adult nucleus and embryonic lens was achieved by fractionation on DEAE-cellulose columns. Electrophoretic analysis of the purified γ-crystallin showed that those of the embryo and adult nucleus have similar mobilities whereas the mobility of adult cortex γ-crystallins differed from the others. It appears, therefore that the γ-crystallins formed in fiber cell differentiation in an embryonic lens are electrophoretically distinct from those formed during fiber differentiation in an adult lens.
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U2 - 10.1016/0304-4165(65)90390-9
DO - 10.1016/0304-4165(65)90390-9
M3 - Article
C2 - 5857371
AN - SCOPUS:0013853310
SN - 0304-4165
VL - 107
SP - 81
EP - 90
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 1
ER -