TY - JOUR
T1 - Biliary endogenous inorganic phosphate, D-glucose, IgA and transferrin are differentially altered by hydrostatic pressure
AU - Moslen, Mary Treinen
AU - Kanz, Mary F.
AU - Bhatia, Jatinder
AU - Kaphalia, Lata
AU - Goldblum, Randall M.
N1 - Funding Information:
This research was supported by NIH Grants: DK 34806, ES 04273, HD 22828 and HD 21412.
PY - 1992
Y1 - 1992
N2 - Our objective was to determine the effects of hydrostatic biliary pressure on excretion patterns of endogenous solutes which reflect various pathways of bile formation. A stable in vivo model was developed using anesthetized rats intraduodenally infused with taurocholate to maintain bile flow. Bile was collected during a 2-h basal period, a 4-h pressure period where elevation of the bile duct cannula decreased bile flow to 1/3 the basal rate, and a 2-h period after release of hydrostatic biliary pressure. During pressure treatment, bile salt concentration gradually increased ∼3-fold, biliary inorganic phosphate concentrations rapidly rost ∼5-fold, and biliary glucose concentration progressively rose ∼17-fold. Concentrations of proteins in bile were affected differently with extreme decreases in IgA, moderate decreases in total protein and leucine aminopeptidase, and minimal change in transferrin. By 2 h after pressure release, only the alterations in biliary glucose and IgA persisted. The observed striking and persisting increases in biliary glucose are tentatively explained as an impaired reabsorption of glucose by the biliary tract.
AB - Our objective was to determine the effects of hydrostatic biliary pressure on excretion patterns of endogenous solutes which reflect various pathways of bile formation. A stable in vivo model was developed using anesthetized rats intraduodenally infused with taurocholate to maintain bile flow. Bile was collected during a 2-h basal period, a 4-h pressure period where elevation of the bile duct cannula decreased bile flow to 1/3 the basal rate, and a 2-h period after release of hydrostatic biliary pressure. During pressure treatment, bile salt concentration gradually increased ∼3-fold, biliary inorganic phosphate concentrations rapidly rost ∼5-fold, and biliary glucose concentration progressively rose ∼17-fold. Concentrations of proteins in bile were affected differently with extreme decreases in IgA, moderate decreases in total protein and leucine aminopeptidase, and minimal change in transferrin. By 2 h after pressure release, only the alterations in biliary glucose and IgA persisted. The observed striking and persisting increases in biliary glucose are tentatively explained as an impaired reabsorption of glucose by the biliary tract.
KW - Biliary permeability
KW - Biliary reabsorption
KW - Cholestasis
KW - Paracellular pathway
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U2 - 10.1016/S0168-8278(05)80099-6
DO - 10.1016/S0168-8278(05)80099-6
M3 - Article
C2 - 1484172
AN - SCOPUS:0026677082
SN - 0168-8278
VL - 16
SP - 89
EP - 97
JO - Journal of hepatology
JF - Journal of hepatology
IS - 1-2
ER -