TY - JOUR
T1 - Bacterial proteomics and vaccine development.
AU - Nilsson, Carol L.
N1 - Funding Information:
This article is dedicated to Professor Emeritus Karl-Anders Karlsson, who introduced the author to the study of host-microbe interactions and bacterial proteomics. Also, generous financial support from Vetenskapsrådet-Medicin (#14113), the Swedish Society for Medical Research, the Swedish Society of Medicine and the Strategic Research Foundation (Infection & Vaccinology) for the work performed in this laboratory is gratefully acknowledged.
PY - 2002
Y1 - 2002
N2 - Until recently, the development of vaccines for use in humans relied on the response to attenuated or whole-cell preparations, or empirically selected antigens. The post-genomic era holds the possibility of rational design of novel vaccines for important human pathogens. The discovery and development of these new vaccines is likely to be accomplished through integrated proteomic strategies. Although most proteomic studies are based on two-dimensional gel electrophoresis (2D-PAGE) as a separation technique, new methods have been developed within the past two years that provide complementary information concerning microbial protein expression. The 2D-PAGE technique in combination with Western blotting has been successfully applied in the discovery of antigens from Helicobacter pylori, Chlamydia trachomatis and Borrelia garinii. Two-dimensional semi-preparative electrophoresis has provided complementary information regarding membrane protein expression in a strain of H. pylori. Through two-dimensional liquid chromatography-tandem mass spectrometry, the most comprehensive information to date regarding protein expression in yeast was obtained. This technique may shortly become an important tool in vaccinology. This review of the current state of bacterial proteomics as applied in vaccinology presents analytical techniques for protein separation, proteomics without gels, reverse vaccinology, and functional approaches to the identification of virulence proteins in microbes.
AB - Until recently, the development of vaccines for use in humans relied on the response to attenuated or whole-cell preparations, or empirically selected antigens. The post-genomic era holds the possibility of rational design of novel vaccines for important human pathogens. The discovery and development of these new vaccines is likely to be accomplished through integrated proteomic strategies. Although most proteomic studies are based on two-dimensional gel electrophoresis (2D-PAGE) as a separation technique, new methods have been developed within the past two years that provide complementary information concerning microbial protein expression. The 2D-PAGE technique in combination with Western blotting has been successfully applied in the discovery of antigens from Helicobacter pylori, Chlamydia trachomatis and Borrelia garinii. Two-dimensional semi-preparative electrophoresis has provided complementary information regarding membrane protein expression in a strain of H. pylori. Through two-dimensional liquid chromatography-tandem mass spectrometry, the most comprehensive information to date regarding protein expression in yeast was obtained. This technique may shortly become an important tool in vaccinology. This review of the current state of bacterial proteomics as applied in vaccinology presents analytical techniques for protein separation, proteomics without gels, reverse vaccinology, and functional approaches to the identification of virulence proteins in microbes.
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U2 - 10.2165/00129785-200202010-00005
DO - 10.2165/00129785-200202010-00005
M3 - Review article
C2 - 12083954
AN - SCOPUS:0036046826
SN - 1175-2203
VL - 2
SP - 59
EP - 65
JO - American journal of pharmacogenomics : genomics-related research in drug development and clinical practice
JF - American journal of pharmacogenomics : genomics-related research in drug development and clinical practice
IS - 1
ER -