TY - JOUR
T1 - Association of hyperglycemia mediated increased advanced glycation and erythrocyte antioxidant enzyme activity in different stages of diabetic retinopathy
AU - Choudhuri, Subhadip
AU - Dutta, Deep
AU - Chowdhury, Imran H.
AU - Mitra, Bhaskar
AU - Sen, Aditi
AU - Mandal, Lakshmi K.
AU - Mukhopadhyay, Satinath
AU - Bhattacharya, Basudev
N1 - Funding Information:
This work was supported by an intramural grant from Indian Council of Medical Research (ICMR), Government of India .
PY - 2013/6
Y1 - 2013/6
N2 - Aim: This study aimed to evaluate whether hyperglycemia mediated increased formation of advanced glycation end products (AGEs) was associated with erythrocyte antioxidant enzyme activity in subjects with different stages of diabetic retinopathy (DR). Methods: Serum level of AGEs was determined by enzyme linked immunosorbent assay. Erythrocyte superoxide dismutase (SOD), glutathione reductase (GR) and catalase activity were estimated by enzymatic reaction based spectrophotometric assay in patients with type 2 diabetes with proliferative diabetic retinopathy (PDR), non-proliferative diabetic retinopathy (NPDR) and no retinopathy (DNR) and also in healthy non-diabetic controls (HC). Result: Erythrocyte SOD and GR activity was significantly lower among NPDR (p= 0.024, 0.0017, respectively) and PDR (p= 0.0003, 0.0001, respectively) subjects compared with DNR individuals. A significant inverse correlation was observed between serum AGEs and erythrocyte SOD or GR activity in DNR (p= 0.0019; r= -0.3033, p= 0.0021; r= -0.3015, respectively), NPDR (p= 0.0001; r= -0.4602, p= 0.0003; r= -0.4161, respectively), and PDR (p<. 0.0001; r= -0.6753, p<. 0.0001; r= -0.5854, respectively) individuals. Conclusion: Poor glycemia may be the key factor enhancing AGE formation, which may be associated with lower erythrocyte SOD and GR activity along with increased catalase activity in DR.
AB - Aim: This study aimed to evaluate whether hyperglycemia mediated increased formation of advanced glycation end products (AGEs) was associated with erythrocyte antioxidant enzyme activity in subjects with different stages of diabetic retinopathy (DR). Methods: Serum level of AGEs was determined by enzyme linked immunosorbent assay. Erythrocyte superoxide dismutase (SOD), glutathione reductase (GR) and catalase activity were estimated by enzymatic reaction based spectrophotometric assay in patients with type 2 diabetes with proliferative diabetic retinopathy (PDR), non-proliferative diabetic retinopathy (NPDR) and no retinopathy (DNR) and also in healthy non-diabetic controls (HC). Result: Erythrocyte SOD and GR activity was significantly lower among NPDR (p= 0.024, 0.0017, respectively) and PDR (p= 0.0003, 0.0001, respectively) subjects compared with DNR individuals. A significant inverse correlation was observed between serum AGEs and erythrocyte SOD or GR activity in DNR (p= 0.0019; r= -0.3033, p= 0.0021; r= -0.3015, respectively), NPDR (p= 0.0001; r= -0.4602, p= 0.0003; r= -0.4161, respectively), and PDR (p<. 0.0001; r= -0.6753, p<. 0.0001; r= -0.5854, respectively) individuals. Conclusion: Poor glycemia may be the key factor enhancing AGE formation, which may be associated with lower erythrocyte SOD and GR activity along with increased catalase activity in DR.
KW - Advanced glycation end products
KW - Catalase
KW - Glutathione reductase
KW - Non-proliferative diabetic retinopathy
KW - Proliferative diabetic retinopathy
KW - Superoxide dismutase
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U2 - 10.1016/j.diabres.2013.03.031
DO - 10.1016/j.diabres.2013.03.031
M3 - Article
C2 - 23602454
AN - SCOPUS:84878472915
SN - 0168-8227
VL - 100
SP - 376
EP - 384
JO - Diabetes Research and Clinical Practice
JF - Diabetes Research and Clinical Practice
IS - 3
ER -