Application of the enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of schistosoma mansoni infections in St. Lucia

Moira L. McLaren; E. G. Long; R. W. Goodgame; Jane E. Lillywhite

doi:10.1016/0035-9203(79)90009-9

Application of the enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of schistosoma mansoni infections in St. Lucia

Moira L. McLaren, E. G. Long, R. W. Goodgame, Jane E. Lillywhite

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

As part of our search for a serodiagnostic assay to replace the expensive and tedious stool examination in the diagnosis of Schistosoma mansoni infection, we have studied the sensitivity, specificity and quantitative features of an enzyme linked immuno-assay (ELISA) using crude S. mansoni egg antigen preparation. Results of studies carried out in both London and St. Lucia indicate that the assay can give useful serodiagnostic information ranging from 82 to 99. 5% sensitivity, depending on level of infection intensity and method of blood collection and 100% specificity in St. Lucian/St. Vincent populations. The St. Lucia study also showed that the assay could be operated in a qualitative form in an endemic area.

Original language	English (US)
Pages (from-to)	636-639
Number of pages	4
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	73
Issue number	6
DOIs	https://doi.org/10.1016/0035-9203(79)90009-9
State	Published - 1979
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/0035-9203(79)90009-9

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title = "Application of the enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of schistosoma mansoni infections in St. Lucia",

abstract = "As part of our search for a serodiagnostic assay to replace the expensive and tedious stool examination in the diagnosis of Schistosoma mansoni infection, we have studied the sensitivity, specificity and quantitative features of an enzyme linked immuno-assay (ELISA) using crude S. mansoni egg antigen preparation. Results of studies carried out in both London and St. Lucia indicate that the assay can give useful serodiagnostic information ranging from 82 to 99. 5% sensitivity, depending on level of infection intensity and method of blood collection and 100% specificity in St. Lucian/St. Vincent populations. The St. Lucia study also showed that the assay could be operated in a qualitative form in an endemic area.",

author = "McLaren, {Moira L.} and Long, {E. G.} and Goodgame, {R. W.} and Lillywhite, {Jane E.}",

note = "Funding Information: Acknowledgements We would like to thank Dr. P. Jordan and Dr. C. C. Draper for helpful advice and encouragement. This investigation received financial support from the UNDPWorld Bank/WHO Soecial Programme for Research and Training in Tropical Diseases. and the Edna McConnell Clark Foundation.",

year = "1979",

doi = "10.1016/0035-9203(79)90009-9",

language = "English (US)",

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pages = "636--639",

journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",

issn = "0035-9203",

publisher = "Oxford University Press",

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T1 - Application of the enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of schistosoma mansoni infections in St. Lucia

AU - McLaren, Moira L.

AU - Long, E. G.

AU - Goodgame, R. W.

AU - Lillywhite, Jane E.

N1 - Funding Information: Acknowledgements We would like to thank Dr. P. Jordan and Dr. C. C. Draper for helpful advice and encouragement. This investigation received financial support from the UNDPWorld Bank/WHO Soecial Programme for Research and Training in Tropical Diseases. and the Edna McConnell Clark Foundation.

PY - 1979

Y1 - 1979

N2 - As part of our search for a serodiagnostic assay to replace the expensive and tedious stool examination in the diagnosis of Schistosoma mansoni infection, we have studied the sensitivity, specificity and quantitative features of an enzyme linked immuno-assay (ELISA) using crude S. mansoni egg antigen preparation. Results of studies carried out in both London and St. Lucia indicate that the assay can give useful serodiagnostic information ranging from 82 to 99. 5% sensitivity, depending on level of infection intensity and method of blood collection and 100% specificity in St. Lucian/St. Vincent populations. The St. Lucia study also showed that the assay could be operated in a qualitative form in an endemic area.

AB - As part of our search for a serodiagnostic assay to replace the expensive and tedious stool examination in the diagnosis of Schistosoma mansoni infection, we have studied the sensitivity, specificity and quantitative features of an enzyme linked immuno-assay (ELISA) using crude S. mansoni egg antigen preparation. Results of studies carried out in both London and St. Lucia indicate that the assay can give useful serodiagnostic information ranging from 82 to 99. 5% sensitivity, depending on level of infection intensity and method of blood collection and 100% specificity in St. Lucian/St. Vincent populations. The St. Lucia study also showed that the assay could be operated in a qualitative form in an endemic area.

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Application of the enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of schistosoma mansoni infections in St. Lucia

Abstract

ASJC Scopus subject areas

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