Androgens and brain differentiation. Effects of testosterone on protein synthesis in neonatal rat hypothalamic and cerebral slices

Protein synthetic activity of anterior hypothalamic and neocortical brain slices from androgenized neonatal female rats was studied in vitro in an attempt to clarify the mechanism of action of testosterone on the developing hypothalamus. The short-term effects of testosterone proprionate injections of the animals on the protein synthetic activity of the slices were assayed by means of incorporation of ¹⁴C-leucine into acid-insoluble protein. Although a high dose (1 mg/animal) of testosterone proprionate stimulated increased incorporation in neocortical and anterior hypothalamic slices, a lower dose and other approaches failed to demonstrate a stimulation regionally specific to the anterior hypothalamus. It is concluded that if testosterone has specific stimulatory effects on protein synthesis in the anterior hypothalamus of neonatally androgenized rats, these are not readily demonstrable by methods used with other steroid target tissues.