An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope

Jing Jin; Michael B. Sherman; Daniel Chafets; Nuntana Dinglasan; Kai Lu; Tzong Hae Lee; Lars Anders Carlson; Marcus O. Muench; Graham Simmons

doi:10.1371/journal.pntd.0006693

An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope

Jing Jin, Michael B. Sherman, Daniel Chafets, Nuntana Dinglasan, Kai Lu, Tzong Hae Lee, Lars Anders Carlson, Marcus O. Muench, Graham Simmons

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Background: Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. Methodology/Principal findings: To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. Conclusions/Significance: Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases.

Original language	English (US)
Article number	e0006693
Journal	PLoS neglected tropical diseases
Volume	12
Issue number	7
DOIs	https://doi.org/10.1371/journal.pntd.0006693
State	Published - Jul 31 2018

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Infectious Diseases

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title = "An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope",

abstract = "Background: Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. Methodology/Principal findings: To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. Conclusions/Significance: Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases.",

author = "Jing Jin and Sherman, {Michael B.} and Daniel Chafets and Nuntana Dinglasan and Kai Lu and Lee, {Tzong Hae} and Carlson, {Lars Anders} and Muench, {Marcus O.} and Graham Simmons",

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AU - Jin, Jing

AU - Sherman, Michael B.

AU - Chafets, Daniel

AU - Dinglasan, Nuntana

AU - Lu, Kai

AU - Lee, Tzong Hae

AU - Carlson, Lars Anders

AU - Muench, Marcus O.

AU - Simmons, Graham

PY - 2018/7/31

Y1 - 2018/7/31

N2 - Background: Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. Methodology/Principal findings: To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. Conclusions/Significance: Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases.

AB - Background: Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. Methodology/Principal findings: To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. Conclusions/Significance: Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases.

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An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope

Abstract

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