Alterations in leukocyte adhesion molecule expression after burn injury

F. E. Nwariaku; W. J. Mileski; E. Lightfoot; P. J. Sikes; P. E. Lipsky

doi:10.1097/00005373-199508000-00016

Alterations in leukocyte adhesion molecule expression after burn injury

F. E. Nwariaku, W. J. Mileski, E. Lightfoot, P. J. Sikes, P. E. Lipsky

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Objective: To determine if thermal injury alters the expression of leukocyte adhesion molecules. Design: This is a controlled experimental animal study. Materials and Methods: Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4. Measurements and Main Results: ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03). Conclusion: Thermal injury alters the expression of leukocyte adhesion molecules.

Original language	English (US)
Pages (from-to)	285-288
Number of pages	4
Journal	Journal of Trauma - Injury, Infection and Critical Care
Volume	39
Issue number	2
DOIs	https://doi.org/10.1097/00005373-199508000-00016
State	Published - 1995
Externally published	Yes

Keywords

Burns
Inflammation
Injury
Integrins
Intercellular adhesion molecule (ICAM)
Neutrophil
Selectins

ASJC Scopus subject areas

Surgery

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10.1097/00005373-199508000-00016

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@article{ff645d02edef40b0a9716982d666fc46,

title = "Alterations in leukocyte adhesion molecule expression after burn injury",

abstract = "Objective: To determine if thermal injury alters the expression of leukocyte adhesion molecules. Design: This is a controlled experimental animal study. Materials and Methods: Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4. Measurements and Main Results: ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03). Conclusion: Thermal injury alters the expression of leukocyte adhesion molecules.",

keywords = "Burns, Inflammation, Injury, Integrins, Intercellular adhesion molecule (ICAM), Neutrophil, Selectins",

author = "Nwariaku, {F. E.} and Mileski, {W. J.} and E. Lightfoot and Sikes, {P. J.} and Lipsky, {P. E.}",

year = "1995",

doi = "10.1097/00005373-199508000-00016",

language = "English (US)",

volume = "39",

pages = "285--288",

journal = "Journal of Trauma - Injury, Infection and Critical Care",

issn = "0022-5282",

publisher = "Lippincott Williams and Wilkins",

number = "2",

}

TY - JOUR

T1 - Alterations in leukocyte adhesion molecule expression after burn injury

AU - Nwariaku, F. E.

AU - Mileski, W. J.

AU - Lightfoot, E.

AU - Sikes, P. J.

AU - Lipsky, P. E.

PY - 1995

Y1 - 1995

N2 - Objective: To determine if thermal injury alters the expression of leukocyte adhesion molecules. Design: This is a controlled experimental animal study. Materials and Methods: Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4. Measurements and Main Results: ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03). Conclusion: Thermal injury alters the expression of leukocyte adhesion molecules.

AB - Objective: To determine if thermal injury alters the expression of leukocyte adhesion molecules. Design: This is a controlled experimental animal study. Materials and Methods: Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4. Measurements and Main Results: ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03). Conclusion: Thermal injury alters the expression of leukocyte adhesion molecules.

KW - Burns

KW - Inflammation

KW - Injury

KW - Integrins

KW - Intercellular adhesion molecule (ICAM)

KW - Neutrophil

KW - Selectins

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SN - 0022-5282

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JF - Journal of Trauma - Injury, Infection and Critical Care

IS - 2

ER -

Alterations in leukocyte adhesion molecule expression after burn injury

Abstract

Keywords

ASJC Scopus subject areas

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