Aldose reductase regulates TNF-α-induced PGE2 production in human colon cancer cells

Ravinder Tammali; Kota V. Ramana; Satish K. Srivastava

doi:10.1016/j.canlet.2007.01.001

Aldose reductase regulates TNF-α-induced PGE2 production in human colon cancer cells

Ravinder Tammali, Kota V. Ramana, Satish K. Srivastava

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Pro-inflammatory cytokines such as TNF-α play an important role in the pathophysiology of diseases such as Crohn's and ulcerative colitis which cause increased risk of colorectal cancer. However, the mechanisms underlying colon carcinogenesis are not well understood. Herein we report that inhibition/antisense abolition of polyol pathway enzyme, aldose reductase (AR) inhibited the TNF-α-induced synthesis of prostaglandin E2 and the activity of cyclooxygenase (Cox) in human colon cancer cells, Caco-2. Inhibition of AR prevented TNF-α-induced activation of PKC and NF-κB which resulted in the abrogation of Cox-2 mRNA and protein expression. These results suggest that inhibition of AR could be a novel chemopreventive approach to colon cancer.

Original language	English (US)
Pages (from-to)	299-306
Number of pages	8
Journal	Cancer Letters
Volume	252
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2007.01.001
State	Published - Jul 18 2007
Externally published	Yes

Keywords

Aldose reductase
Colon cancer
Inflammation
NF-κB
PGE2
PKC
TNF-α

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/j.canlet.2007.01.001

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@article{c09dc226b2404ade900e44fca49bd9f5,

title = "Aldose reductase regulates TNF-α-induced PGE2 production in human colon cancer cells",

abstract = "Pro-inflammatory cytokines such as TNF-α play an important role in the pathophysiology of diseases such as Crohn's and ulcerative colitis which cause increased risk of colorectal cancer. However, the mechanisms underlying colon carcinogenesis are not well understood. Herein we report that inhibition/antisense abolition of polyol pathway enzyme, aldose reductase (AR) inhibited the TNF-α-induced synthesis of prostaglandin E2 and the activity of cyclooxygenase (Cox) in human colon cancer cells, Caco-2. Inhibition of AR prevented TNF-α-induced activation of PKC and NF-κB which resulted in the abrogation of Cox-2 mRNA and protein expression. These results suggest that inhibition of AR could be a novel chemopreventive approach to colon cancer.",

keywords = "Aldose reductase, Colon cancer, Inflammation, NF-κB, PGE2, PKC, TNF-α",

author = "Ravinder Tammali and Ramana, {Kota V.} and Srivastava, {Satish K.}",

note = "Funding Information: This work was supported in part by NIH Grants GM 71036 (to K.V.R.), and DK 36118 (to S.K.S.).",

year = "2007",

month = jul,

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doi = "10.1016/j.canlet.2007.01.001",

language = "English (US)",

volume = "252",

pages = "299--306",

journal = "Cancer Letters",

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publisher = "Elsevier Ireland Ltd",

number = "2",

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TY - JOUR

T1 - Aldose reductase regulates TNF-α-induced PGE2 production in human colon cancer cells

AU - Tammali, Ravinder

AU - Ramana, Kota V.

AU - Srivastava, Satish K.

N1 - Funding Information: This work was supported in part by NIH Grants GM 71036 (to K.V.R.), and DK 36118 (to S.K.S.).

PY - 2007/7/18

Y1 - 2007/7/18

N2 - Pro-inflammatory cytokines such as TNF-α play an important role in the pathophysiology of diseases such as Crohn's and ulcerative colitis which cause increased risk of colorectal cancer. However, the mechanisms underlying colon carcinogenesis are not well understood. Herein we report that inhibition/antisense abolition of polyol pathway enzyme, aldose reductase (AR) inhibited the TNF-α-induced synthesis of prostaglandin E2 and the activity of cyclooxygenase (Cox) in human colon cancer cells, Caco-2. Inhibition of AR prevented TNF-α-induced activation of PKC and NF-κB which resulted in the abrogation of Cox-2 mRNA and protein expression. These results suggest that inhibition of AR could be a novel chemopreventive approach to colon cancer.

AB - Pro-inflammatory cytokines such as TNF-α play an important role in the pathophysiology of diseases such as Crohn's and ulcerative colitis which cause increased risk of colorectal cancer. However, the mechanisms underlying colon carcinogenesis are not well understood. Herein we report that inhibition/antisense abolition of polyol pathway enzyme, aldose reductase (AR) inhibited the TNF-α-induced synthesis of prostaglandin E2 and the activity of cyclooxygenase (Cox) in human colon cancer cells, Caco-2. Inhibition of AR prevented TNF-α-induced activation of PKC and NF-κB which resulted in the abrogation of Cox-2 mRNA and protein expression. These results suggest that inhibition of AR could be a novel chemopreventive approach to colon cancer.

KW - Aldose reductase

KW - Colon cancer

KW - Inflammation

KW - NF-κB

KW - PGE2

KW - PKC

KW - TNF-α

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U2 - 10.1016/j.canlet.2007.01.001

DO - 10.1016/j.canlet.2007.01.001

M3 - Article

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AN - SCOPUS:34249732513

SN - 0304-3835

VL - 252

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EP - 306

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Aldose reductase regulates TNF-α-induced PGE2 production in human colon cancer cells

Abstract

Keywords

ASJC Scopus subject areas

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