Agglutination of bacteria and erythrocytes by serum from six species of marine molluscs

William S. Fisher; Anthony R. DiNuzzo

doi:10.1016/0022-2011(91)90142-D

Agglutination of bacteria and erythrocytes by serum from six species of marine molluscs

William S. Fisher, Anthony R. DiNuzzo

Internal Medicine

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Lectins have been implicated as recognition molecules in the invertebrate immune response, yet their capacity to recognize (agglutinate and/or opsonize) potentially invasive microorganisms is largely unknown. In this study, sera from six species of marine molluscs (oyster, clam, octopus, squid, cuttlefish, and sea hare) were found to agglutinate 64 of 94 bacterial isolates (15 genera, 36 species) and seven types of vertebrate erythrocytes. Oyster, clam, and octopus sera agglutinated the greatest number of bacterial isolates and oyster serum exhibited the highest intensity and titer of agglutination. Agglutination was isolate dependent, implying high lectin specificity. Titers were highly variable for wild populations of oysters and sea hares and relatively constant for F4-generation cuttlefish reared in aquaculture systems. Simultaneous agglutination of specific bacterial isolates indicated conservation of lectin specificity between oyster and clam sera (bivalves) and between squid and cuttlefish sera (cephalopods).

Original language	English (US)
Pages (from-to)	380-394
Number of pages	15
Journal	Journal of Invertebrate Pathology
Volume	57
Issue number	3
DOIs	https://doi.org/10.1016/0022-2011(91)90142-D
State	Published - May 1991

Keywords

Agglutination
hemagglutination
invertebrate immunology
lectins
marine molluscs
nonself recognition

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

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10.1016/0022-2011(91)90142-D

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title = "Agglutination of bacteria and erythrocytes by serum from six species of marine molluscs",

abstract = "Lectins have been implicated as recognition molecules in the invertebrate immune response, yet their capacity to recognize (agglutinate and/or opsonize) potentially invasive microorganisms is largely unknown. In this study, sera from six species of marine molluscs (oyster, clam, octopus, squid, cuttlefish, and sea hare) were found to agglutinate 64 of 94 bacterial isolates (15 genera, 36 species) and seven types of vertebrate erythrocytes. Oyster, clam, and octopus sera agglutinated the greatest number of bacterial isolates and oyster serum exhibited the highest intensity and titer of agglutination. Agglutination was isolate dependent, implying high lectin specificity. Titers were highly variable for wild populations of oysters and sea hares and relatively constant for F4-generation cuttlefish reared in aquaculture systems. Simultaneous agglutination of specific bacterial isolates indicated conservation of lectin specificity between oyster and clam sera (bivalves) and between squid and cuttlefish sera (cephalopods).",

keywords = "Agglutination, hemagglutination, invertebrate immunology, lectins, marine molluscs, nonself recognition",

author = "Fisher, {William S.} and DiNuzzo, {Anthony R.}",

note = "Funding Information: i This investigation was supported by funds from the University of Texas Medical Branch Small Grants Program and National Institutes of Health (Depart- ment of Health and Human Services) Grants RR01024 and RR04226. * To whom reprint requests should be addressed. Funding Information: We thank all those colleagues who contributed bacteria isolates and mollusc serum, staff members of the Marine Biomedical Institute for collecting and maintaining animals and sampling hemolymph, Inthumathi Baskaran for technical assistance, and Dr. M. L. Tam-plin for his critical review of the manuscript. This work was supported by funds from the University of Texas Medical Branch and by grants from the National Institutes of Health No. RR01024 and No. RR04226.",

year = "1991",

month = may,

doi = "10.1016/0022-2011(91)90142-D",

language = "English (US)",

volume = "57",

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journal = "Journal of Invertebrate Pathology",

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AU - Fisher, William S.

AU - DiNuzzo, Anthony R.

N1 - Funding Information: i This investigation was supported by funds from the University of Texas Medical Branch Small Grants Program and National Institutes of Health (Depart- ment of Health and Human Services) Grants RR01024 and RR04226. * To whom reprint requests should be addressed. Funding Information: We thank all those colleagues who contributed bacteria isolates and mollusc serum, staff members of the Marine Biomedical Institute for collecting and maintaining animals and sampling hemolymph, Inthumathi Baskaran for technical assistance, and Dr. M. L. Tam-plin for his critical review of the manuscript. This work was supported by funds from the University of Texas Medical Branch and by grants from the National Institutes of Health No. RR01024 and No. RR04226.

PY - 1991/5

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N2 - Lectins have been implicated as recognition molecules in the invertebrate immune response, yet their capacity to recognize (agglutinate and/or opsonize) potentially invasive microorganisms is largely unknown. In this study, sera from six species of marine molluscs (oyster, clam, octopus, squid, cuttlefish, and sea hare) were found to agglutinate 64 of 94 bacterial isolates (15 genera, 36 species) and seven types of vertebrate erythrocytes. Oyster, clam, and octopus sera agglutinated the greatest number of bacterial isolates and oyster serum exhibited the highest intensity and titer of agglutination. Agglutination was isolate dependent, implying high lectin specificity. Titers were highly variable for wild populations of oysters and sea hares and relatively constant for F4-generation cuttlefish reared in aquaculture systems. Simultaneous agglutination of specific bacterial isolates indicated conservation of lectin specificity between oyster and clam sera (bivalves) and between squid and cuttlefish sera (cephalopods).

AB - Lectins have been implicated as recognition molecules in the invertebrate immune response, yet their capacity to recognize (agglutinate and/or opsonize) potentially invasive microorganisms is largely unknown. In this study, sera from six species of marine molluscs (oyster, clam, octopus, squid, cuttlefish, and sea hare) were found to agglutinate 64 of 94 bacterial isolates (15 genera, 36 species) and seven types of vertebrate erythrocytes. Oyster, clam, and octopus sera agglutinated the greatest number of bacterial isolates and oyster serum exhibited the highest intensity and titer of agglutination. Agglutination was isolate dependent, implying high lectin specificity. Titers were highly variable for wild populations of oysters and sea hares and relatively constant for F4-generation cuttlefish reared in aquaculture systems. Simultaneous agglutination of specific bacterial isolates indicated conservation of lectin specificity between oyster and clam sera (bivalves) and between squid and cuttlefish sera (cephalopods).

KW - Agglutination

KW - hemagglutination

KW - invertebrate immunology

KW - lectins

KW - marine molluscs

KW - nonself recognition

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Agglutination of bacteria and erythrocytes by serum from six species of marine molluscs

Abstract

Keywords

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