Abstract
Serial passaging of yellow fever virus 17D in Vero cells was employed to derive seed material for a novel inactivated vaccine, XRX-001. Two independent passaging series identified a novel lysine to arginine mutation at amino acid 160 of the envelope protein, a surface-exposed residue in structural domain I. A third passage series resulted in an isoleucine to methionine mutation at residue 113 of the NS4B protein, a central membrane spanning region of the protein which has previously been associated with Vero cell adaptation of other mosquito-borne flaviviruses. These studies confirm that flavivirus adaptation to growth in Vero cells can be mediated by structural or non-structural protein mutations.
Original language | English (US) |
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Pages (from-to) | 280-284 |
Number of pages | 5 |
Journal | Virus Research |
Volume | 176 |
Issue number | 1-2 |
DOIs | |
State | Published - Sep 2013 |
Keywords
- Vaccine
- Vero cells
- Yellow fever virus
ASJC Scopus subject areas
- Infectious Diseases
- Cancer Research
- Virology