Abstract
Vital staining with the fluorescent dye, acridine orange, was evaluated as a means of detecting abnormalities of lysosomes in cultivated fibroblasts of patients with macular corneal dystrophy and mucopolysaccharidoses types I-H (Hurler's syndrome) and type II (Hunter's syndrome). Multiple cultures were compared with normal fibroblasts using a 'double-masked' design to exclude observer bias. Cells of patients with the mucopolysaccharidoses were easily and accurately separated from other fibroblasts. Contrary to a recent report, corneal fibroblasts of patients with macular corneal dystrophy were indistinguishable from control cells.
Original language | English (US) |
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Pages (from-to) | 297-299 |
Number of pages | 3 |
Journal | Archives of Pathology and Laboratory Medicine |
Volume | 103 |
Issue number | 6 |
State | Published - 1979 |
Externally published | Yes |
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medical Laboratory Technology