Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver

Sun Yee Kim; Choon Kiat Sim; Hui Tang; Weiping Han; Kangling Zhang; Feng Xu

doi:10.1371/journal.pone.0140619

Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver

Sun Yee Kim, Choon Kiat Sim, Hui Tang, Weiping Han, Kangling Zhang, Feng Xu

Pharmacology & Toxicology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Lysine acetylation is a post-translational modification found on numerous proteins, a strategy used in cell signaling to change protein activity in response to internal or external cues. Sirtuin 1 (SIRT1) is a central lysine deacetylase involved in a variety of cellular processes including metabolism, apoptosis, and DNA repair. Here we characterize the lysine acetylome in mouse liver, and by using a model of Sirt1^-/-knockout mouse, show that SIRT1 regulates the deacetylation of 70 proteins in the liver in-vivo. Amongst these SIRT1-regulated proteins, we find that four RNA-processing proteins and a chromatin-remodeling protein can be deacetylated by SIRT1 directly in-vitro. The discovery that SIRT1 has a potential role in RNA-processing suggests a new layer of regulation in the variety of functions performed by SIRT1.

Original language	English (US)
Article number	e0140619
Journal	PloS one
Volume	10
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0140619
State	Published - Oct 15 2015

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abstract = "Lysine acetylation is a post-translational modification found on numerous proteins, a strategy used in cell signaling to change protein activity in response to internal or external cues. Sirtuin 1 (SIRT1) is a central lysine deacetylase involved in a variety of cellular processes including metabolism, apoptosis, and DNA repair. Here we characterize the lysine acetylome in mouse liver, and by using a model of Sirt1-/-knockout mouse, show that SIRT1 regulates the deacetylation of 70 proteins in the liver in-vivo. Amongst these SIRT1-regulated proteins, we find that four RNA-processing proteins and a chromatin-remodeling protein can be deacetylated by SIRT1 directly in-vitro. The discovery that SIRT1 has a potential role in RNA-processing suggests a new layer of regulation in the variety of functions performed by SIRT1.",

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AU - Sim, Choon Kiat

AU - Tang, Hui

AU - Han, Weiping

AU - Zhang, Kangling

AU - Xu, Feng

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Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver

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