TY - JOUR
T1 - Acanthamoeba polyphaga mimivirus stability in environmental and clinical substrates
T2 - Implications for virus detection and isolation
AU - Dornas, Fábio P.
AU - Silva, Lorena C.F.
AU - De Almeida, Gabriel M.
AU - Campos, Rafael K.
AU - Boratto, Paulo V.M.
AU - Franco-Luiz, Ana P.M.
AU - La Scola, Bernard
AU - Ferreira, Paulo C.P.
AU - Kroon, Erna G.
AU - Abrahão, Jônatas S.
PY - 2014/2/3
Y1 - 2014/2/3
N2 - Viruses are extremely diverse and abundant and are present in countless environments. Giant viruses of the Megavirales order have emerged as a fascinating research topic for virologists around the world. As evidence of their ubiquity and ecological impact, mimiviruses have been found in multiple environmental samples. However, isolation of these viruses from environmental samples is inefficient, mainly due to methodological limitations and lack of information regarding the interactions between viruses and substrates. In this work, we demonstrate the long-lasting stability of mimivirus in environmental (freshwater and saline water) and hospital (ventilator plastic device tube) substrates, showing the detection of infectious particles after more than 9 months. In addition, an enrichment protocol was implemented that remarkably increased mimivirus detection from all tested substrates, including field tests. Moreover, biological, morphological and genetic tests revealed that the enrichment protocol maintained mimivirus particle integrity. In conclusion, our work demonstrated the stability of APMV in samples of environmental and health interest and proposed a reliable and easy protocol to improve giant virus isolation. The data presented here can guide future giant virus detection and isolation studies. Copyright:
AB - Viruses are extremely diverse and abundant and are present in countless environments. Giant viruses of the Megavirales order have emerged as a fascinating research topic for virologists around the world. As evidence of their ubiquity and ecological impact, mimiviruses have been found in multiple environmental samples. However, isolation of these viruses from environmental samples is inefficient, mainly due to methodological limitations and lack of information regarding the interactions between viruses and substrates. In this work, we demonstrate the long-lasting stability of mimivirus in environmental (freshwater and saline water) and hospital (ventilator plastic device tube) substrates, showing the detection of infectious particles after more than 9 months. In addition, an enrichment protocol was implemented that remarkably increased mimivirus detection from all tested substrates, including field tests. Moreover, biological, morphological and genetic tests revealed that the enrichment protocol maintained mimivirus particle integrity. In conclusion, our work demonstrated the stability of APMV in samples of environmental and health interest and proposed a reliable and easy protocol to improve giant virus isolation. The data presented here can guide future giant virus detection and isolation studies. Copyright:
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U2 - 10.1371/journal.pone.0087811
DO - 10.1371/journal.pone.0087811
M3 - Article
C2 - 24498379
AN - SCOPUS:84896883192
SN - 1932-6203
VL - 9
JO - PloS one
JF - PloS one
IS - 2
M1 - e87811
ER -