A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of machupo virus

Michael Patterson, Takaaki Koma, Alexey Seregin, Cheng Huang, Milagros Miller, Jennifer Smith, Nadezhda E Yun, Jeanon Smith, Slobodan Paessler

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Machupo virus (MACV) is the etiologic agent of Bolivian hemorrhagic fever (BHF). Utilizing a reverse-genetics system recently developed, we report the rescue of a rationally modified recombinant MACV containing a single mutation in the transmembrane region of the glycoprotein. Following challenge of susceptible mice, we identified a significant reduction in virulence in the novel virus. We also identified an instability leading to reversion of the single mutation to a wild-type genotype.

Original languageEnglish (US)
Pages (from-to)10995-10999
Number of pages5
JournalJournal of virology
Volume88
Issue number18
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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