A Single-Dose Intranasal Combination Panebolavirus Vaccine

Delphine C. Malherbe, James Kimble, Caroline Atyeo, Stephanie Fischinger, Michelle Meyer, S. Gabrielle Cody, Matthew Hyde, Galit Alter, Alexander Bukreyev

Research output: Contribution to journalArticlepeer-review


Background. Ebolaviruses Ebola (EBOV), Sudan (SUDV), and Bundibugyo (BDBV) cause severe human disease, which may be accompanied by hemorrhagic syndrome, with high case fatality rates. Monovalent vaccines do not offer cross-protection against these viruses whose endemic areas overlap. Therefore, development of a panebolavirus vaccine is a priority. As a vaccine vector, human parainfluenza virus type 3 (HPIV3) has the advantages of needle-free administration and induction of both systemic and local mucosal antibody responses in the respiratory tract. Methods. To minimize the antivector immunity, genes encoding the HPIV3 envelope proteins F and HN were removed from the vaccine constructs, resulting in expression of only the ebolavirus envelope protein-glycoprotein. These second-generation vaccine constructs were used to develop a combination vaccine against EBOV, SUDV, and BDBV. Results. A single intranasal vaccination of guinea pigs or ferrets with the trivalent combination vaccine elicited humoral responses to each of the targeted ebolaviruses, including binding and neutralizing antibodies, as well as Fc-mediated effector functions. This vaccine protected animals from death and disease caused by lethal challenges with EBOV, SUDV, or BDBV. Conclusions. The combination vaccine elicited protection that was comparable to that induced by the monovalent vaccines, thus demonstrating the value of this combination trivalent vaccine.

Original languageEnglish (US)
Pages (from-to)S648-S659
JournalJournal of Infectious Diseases
StatePublished - Nov 15 2023
Externally publishedYes


  • Ebola virus
  • intranasal vaccination
  • vaccine

ASJC Scopus subject areas

  • General Medicine


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