TY - JOUR
T1 - A sequential staining technique for karyotypic analysis of interspecific somatic cell hybrids
AU - Keherly, Michael J.
AU - Papaconstantinou, John
AU - McCombs, Jerome L.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1993/4
Y1 - 1993/4
N2 - We describe a sequential staining technique for the karyotypic analysis of interspecific mouse X human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. This procedure not only provided a simple distinction between human and mouse chromosomes, but it also allowed the visualization and monitoring of human sequences present in interspecific translocations and subchromosomal fragments.
AB - We describe a sequential staining technique for the karyotypic analysis of interspecific mouse X human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. This procedure not only provided a simple distinction between human and mouse chromosomes, but it also allowed the visualization and monitoring of human sequences present in interspecific translocations and subchromosomal fragments.
UR - http://www.scopus.com/inward/record.url?scp=0027225378&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027225378&partnerID=8YFLogxK
U2 - 10.1006/excr.1993.1098
DO - 10.1006/excr.1993.1098
M3 - Article
C2 - 8482340
AN - SCOPUS:0027225378
SN - 0014-4827
VL - 205
SP - 361
EP - 364
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -