Abstract
Objective: To evaluate a manual method (Cytosphere) for quantifying CD4+ T-cell numbers. Design: Cross-sectional study of HIV-1-seronegative and HIV-1-seropositive individuals evaluated for absolute CD4 counts by both standardized flow cytometric measurements and manual Cytosphere technology using a hemacytometer. Setting: University research hospitals in both the United States and Africa. Patients, participants: Blood specimens from 382 patients were evaluated. These were broken down into 294 samples obtained from HIV-1-seropositive patients and 88 samples obtained from HIV-1-seronegative patients. Interventions: None. Outcome measured: Absolute CD4 cell number. Results: Evaluation of samples obtained from HIV-1 patients in both the United States and Africa demonstrated an overall correlation of the Cytosphere assay with flow cytometry of 0.912 (95% confidence interval, 0.895-0.928; P<0.001). When samples were stratified based on CD4+ T-cell counts determined by flow cytometry, the Cytosphere assay had a 96% predictive value for correctly identifying individuals with CD4 T-cell counts >200×106/l and a 92% predictive value for correctly identifying individuals with CD4 T-cell counts <200×106/l. Conclusions: This assay appears to have the potential for the quantitation of CD4 cells in the limited laboratory facilities in developing countries and to have a strong correlation with standard flow cytometric technology.
Original language | English (US) |
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Pages (from-to) | 1565-1568 |
Number of pages | 4 |
Journal | AIDS |
Volume | 7 |
Issue number | 12 |
State | Published - Dec 1993 |
Externally published | Yes |
Keywords
- CD4+ T cell
- Developing country
- Flow cytometer
- Hemacytometer
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Infectious Diseases