TY - JOUR
T1 - A panel of induced pluripotent stem cells from chimpanzees
T2 - A resource for comparative functional genomics
AU - Romero, Irene Gallego
AU - Pavlovic, Bryan J.
AU - Hernando-Herraez, Irene
AU - Zhou, Xiang
AU - Ward, Michelle C.
AU - Banovich, Nicholas E.
AU - Kagan, Courtney L.
AU - Burnett, Jonathan E.
AU - Huang, Constance H.
AU - Mitrano, Amy
AU - Chavarria, Claudia I.
AU - Ben-Nun, Inbar Friedrich
AU - Li, Yingchun
AU - Sabatini, Karen
AU - Leonardo, Trevor R.
AU - Parast, Mana
AU - Marques-Bonet, Tomas
AU - Laurent, Louise C.
AU - Loring, Jeanne F.
AU - Gilad, Yoav
N1 - Publisher Copyright:
© Gallego Romero et al.
PY - 2015/6/23
Y1 - 2015/6/23
N2 - Comparative genomics studies in primates are restricted due to our limited access to samples. In order to gain better insight into the genetic processes that underlie variation in complex phenotypes in primates, we must have access to faithful model systems for a wide range of cell types. To facilitate this, we generated a panel of 7 fully characterized chimpanzee induced pluripotent stem cell (iPSC) lines derived from healthy donors. To demonstrate the utility of comparative iPSC panels, we collected RNA-sequencing and DNA methylation data from the chimpanzee iPSCs and the corresponding fibroblast lines, as well as from 7 human iPSCs and their source lines, which encompass multiple populations and cell types. We observe much less withinspecies variation in iPSCs than in somatic cells, indicating the reprogramming process erases many inter-individual differences. The low within-species regulatory variation in iPSCs allowed us to identify many novel inter-species regulatory differences of small magnitude.
AB - Comparative genomics studies in primates are restricted due to our limited access to samples. In order to gain better insight into the genetic processes that underlie variation in complex phenotypes in primates, we must have access to faithful model systems for a wide range of cell types. To facilitate this, we generated a panel of 7 fully characterized chimpanzee induced pluripotent stem cell (iPSC) lines derived from healthy donors. To demonstrate the utility of comparative iPSC panels, we collected RNA-sequencing and DNA methylation data from the chimpanzee iPSCs and the corresponding fibroblast lines, as well as from 7 human iPSCs and their source lines, which encompass multiple populations and cell types. We observe much less withinspecies variation in iPSCs than in somatic cells, indicating the reprogramming process erases many inter-individual differences. The low within-species regulatory variation in iPSCs allowed us to identify many novel inter-species regulatory differences of small magnitude.
UR - http://www.scopus.com/inward/record.url?scp=85042249404&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042249404&partnerID=8YFLogxK
U2 - 10.7554/eLife.07103.001
DO - 10.7554/eLife.07103.001
M3 - Article
C2 - 26102527
AN - SCOPUS:85042249404
SN - 2050-084X
VL - 4
SP - 1
EP - 29
JO - eLife
JF - eLife
IS - JUNE 2015
M1 - e07103
ER -