A Novel Fluorogenic Assay for the Detection of Nephrotoxin-Induced Oxidative Stress in Live Cells and Renal Tissue

Kamalika Mukherjee, Tak Ian Chio, Han Gu, Dan L. Sackett, Susan L. Bane, Sanja Sever

Research output: Contribution to journalArticlepeer-review

Abstract

Drug-induced kidney injury frequently leads to aborted clinical trials and drug withdrawals. Sufficiently sensitive sensors capable of detecting mild signs of chemical insult in cell-based screening assays are critical to identifying and eliminating potential toxins in the preclinical stage. Oxidative stress is a common early manifestation of chemical toxicity, and biomolecule carbonylation is an irreversible repercussion of oxidative stress. Here, we present a novel fluorogenic assay using a sensor, TFCH, that responds to biomolecule carbonylation and efficiently detects modest forms of renal injury with much greater sensitivity than standard assays for nephrotoxins. We demonstrate that this sensor can be deployed in live kidney cells and in renal tissue. Our robust assay may help inform preclinical decisions to recall unsafe drug candidates. The application of this sensor in identifying and analyzing diverse pathologies is envisioned.

Original languageEnglish (US)
Pages (from-to)2523-2528
Number of pages6
JournalACS Sensors
Volume6
Issue number7
DOIs
StatePublished - Jul 23 2021
Externally publishedYes

Keywords

  • carbonylated biomolecules
  • fluorogenic assay
  • hydrazine
  • kidney tissue
  • live cells
  • oxidative stress

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

Fingerprint

Dive into the research topics of 'A Novel Fluorogenic Assay for the Detection of Nephrotoxin-Induced Oxidative Stress in Live Cells and Renal Tissue'. Together they form a unique fingerprint.

Cite this