TY - JOUR
T1 - A human gastric cancer cell line possesses a functional receptor for gastrin-releasing peptide
AU - Bold, Richard J.
AU - Kim, Hong Jin
AU - Ishizuka, Jin
AU - Townsend, Courtney M.
AU - Thompson, James C.
N1 - Funding Information:
This work was supported by funding from National Institutes of Health (5R37 DK15241, PO1 DK35608), Walls Medical Research Foundation and American Cancer Society (CB-571).
PY - 1998
Y1 - 1998
N2 - Bombesin (BBS) exhibits diverse biological functions including those of neurotransmitter, regulator of gastrointestinal hormone release, and mitogen. Gastrin-releasing peptide (GRP, the mammalian equivalent of BBS) is found in mucosal cells of the gastric fundus and antrum. We determined whether a human gastric cancer cell line (SIIA) expresses a functional GRP-receptor (GRP-R). BBS increased intracellular calcium ([Ca2+](i)), and a specific GRP-R antagonist, ([D-Phe6, Des-Mef14]-BBS (6-14)-ethylamide), blocked BBS- induced increase in [Ca2+](i). SIIA cells possess GRP-R mRNA by reverse transcriptase-PCR. Furthermore, these cells possess an 80-kDa cell surface protein that specifically binds BBS with two high-binding affinities (K(d1) = 0.6 nM, K(d2) = 6.7 nM). These findings indicate that SIIA cells possess a GRP-R that is capable of physiological signal transduction, though the cellular response remains unknown.
AB - Bombesin (BBS) exhibits diverse biological functions including those of neurotransmitter, regulator of gastrointestinal hormone release, and mitogen. Gastrin-releasing peptide (GRP, the mammalian equivalent of BBS) is found in mucosal cells of the gastric fundus and antrum. We determined whether a human gastric cancer cell line (SIIA) expresses a functional GRP-receptor (GRP-R). BBS increased intracellular calcium ([Ca2+](i)), and a specific GRP-R antagonist, ([D-Phe6, Des-Mef14]-BBS (6-14)-ethylamide), blocked BBS- induced increase in [Ca2+](i). SIIA cells possess GRP-R mRNA by reverse transcriptase-PCR. Furthermore, these cells possess an 80-kDa cell surface protein that specifically binds BBS with two high-binding affinities (K(d1) = 0.6 nM, K(d2) = 6.7 nM). These findings indicate that SIIA cells possess a GRP-R that is capable of physiological signal transduction, though the cellular response remains unknown.
KW - Bombesin
KW - Gastric cancer
KW - Gastrin-releasing peptide
KW - Signal transduction
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U2 - 10.3109/07357909809039748
DO - 10.3109/07357909809039748
M3 - Article
C2 - 9474246
AN - SCOPUS:0031905924
SN - 0735-7907
VL - 16
SP - 12
EP - 17
JO - Cancer Investigation
JF - Cancer Investigation
IS - 1
ER -