A histone acetyltransferase regulates active DNA demethylation in Arabidopsis

Weiqiang Qian; Daisuke Miki; Heng Zhang; Yunhua Liu; Xi Zhang; Kai Tang; Yunchao Kan; Honggui La; Xiaojie Li; Shaofang Li; Xiaohong Zhu; Xiaobing Shi; Kangling Zhang; Olga Pontes; Xuemei Chen; Renyi Liu; Zhizhong Gong; Jian Kang Zhu

doi:10.1126/science.1219416

A histone acetyltransferase regulates active DNA demethylation in Arabidopsis

Weiqiang Qian, Daisuke Miki, Heng Zhang, Yunhua Liu, Xi Zhang, Kai Tang, Yunchao Kan, Honggui La, Xiaojie Li, Shaofang Li, Xiaohong Zhu, Xiaobing Shi, Kangling Zhang, Olga Pontes, Xuemei Chen, Renyi Liu, Zhizhong Gong, Jian Kang Zhu

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Abstract

Active DNA demethylation is an important part of epigenetic regulation in plants and animals. How active DNA demethylation is regulated and its relationship with histone modification patterns are unclear. Here, we report the discovery of IDM1, a regulator of DNA demethylation in Arabidopsis. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function. Our study reveals how some genes are indicated by multiple epigenetic marks for active DNA demethylation and protection from silencing.

Original language	English (US)
Pages (from-to)	1445-1448
Number of pages	4
Journal	Science
Volume	336
Issue number	6087
DOIs	https://doi.org/10.1126/science.1219416
State	Published - Jun 15 2012
Externally published	Yes

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title = "A histone acetyltransferase regulates active DNA demethylation in Arabidopsis",

abstract = "Active DNA demethylation is an important part of epigenetic regulation in plants and animals. How active DNA demethylation is regulated and its relationship with histone modification patterns are unclear. Here, we report the discovery of IDM1, a regulator of DNA demethylation in Arabidopsis. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function. Our study reveals how some genes are indicated by multiple epigenetic marks for active DNA demethylation and protection from silencing.",

author = "Weiqiang Qian and Daisuke Miki and Heng Zhang and Yunhua Liu and Xi Zhang and Kai Tang and Yunchao Kan and Honggui La and Xiaojie Li and Shaofang Li and Xiaohong Zhu and Xiaobing Shi and Kangling Zhang and Olga Pontes and Xuemei Chen and Renyi Liu and Zhizhong Gong and Zhu, {Jian Kang}",

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doi = "10.1126/science.1219416",

language = "English (US)",

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AU - Qian, Weiqiang

AU - Miki, Daisuke

AU - Zhang, Heng

AU - Liu, Yunhua

AU - Zhang, Xi

AU - Tang, Kai

AU - Kan, Yunchao

AU - La, Honggui

AU - Li, Xiaojie

AU - Li, Shaofang

AU - Zhu, Xiaohong

AU - Shi, Xiaobing

AU - Zhang, Kangling

AU - Pontes, Olga

AU - Chen, Xuemei

AU - Liu, Renyi

AU - Gong, Zhizhong

AU - Zhu, Jian Kang

PY - 2012/6/15

Y1 - 2012/6/15

N2 - Active DNA demethylation is an important part of epigenetic regulation in plants and animals. How active DNA demethylation is regulated and its relationship with histone modification patterns are unclear. Here, we report the discovery of IDM1, a regulator of DNA demethylation in Arabidopsis. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function. Our study reveals how some genes are indicated by multiple epigenetic marks for active DNA demethylation and protection from silencing.

AB - Active DNA demethylation is an important part of epigenetic regulation in plants and animals. How active DNA demethylation is regulated and its relationship with histone modification patterns are unclear. Here, we report the discovery of IDM1, a regulator of DNA demethylation in Arabidopsis. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function. Our study reveals how some genes are indicated by multiple epigenetic marks for active DNA demethylation and protection from silencing.

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A histone acetyltransferase regulates active DNA demethylation in Arabidopsis

Abstract

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