Vysokochuvstvitel'naia neizotopnaia sistema gibridizatsii DNK s primeneniem amplifikatsii (PTsR) dlia identifikatsii i indikatsii brutsell.

I. K. Kulakov; V. N. Gorelov; V. L. Motin; G. V. Brukhanskiǐ; A. G. Skavronskaia

Vysokochuvstvitel'naia neizotopnaia sistema gibridizatsii DNK s primeneniem amplifikatsii (PTsR) dlia identifikatsii i indikatsii brutsell.

Translated title of the contribution: A highly sensitive non-isotopic system of DNA hybridization using amplification (PCR) for identifying and indicating presence of Brucella

I. K. Kulakov, V. N. Gorelov, V. L. Motin, G. V. Brukhanskiǐ, A. G. Skavronskaia

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A non-isotopic amplification system was used to identify and indicate Brucella. The terminal sequences of a protein gene fragment in Brucella outer membrane were identified and direct and reverse primers were chosen for a polymerase chain reaction. (PCR). PCR amplifies a specific DNA fragment, 700 kb in size, only in representatives of the Brucella genus. A probe was design, which is the central part of the amplified DNA fragment, 550 kb in size. Single Brucella cells were detectable with an unlabelled probe in the analyzed samples during hybridization reactions. The system can be recommended for a rapid and reliable analysis in medical and veterinary practice.

Translated title of the contribution	A highly sensitive non-isotopic system of DNA hybridization using amplification (PCR) for identifying and indicating presence of Brucella
Original language	Russian
Pages (from-to)	23-27
Number of pages	5
Journal	Molekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number	7-8
State	Published - Jul 1992
Externally published	Yes

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Vysokochuvstvitel'naia neizotopnaia sistema gibridizatsii DNK s primeneniem amplifikatsii (PTsR) dlia identifikatsii i indikatsii brutsell.",

abstract = "A non-isotopic amplification system was used to identify and indicate Brucella. The terminal sequences of a protein gene fragment in Brucella outer membrane were identified and direct and reverse primers were chosen for a polymerase chain reaction. (PCR). PCR amplifies a specific DNA fragment, 700 kb in size, only in representatives of the Brucella genus. A probe was design, which is the central part of the amplified DNA fragment, 550 kb in size. Single Brucella cells were detectable with an unlabelled probe in the analyzed samples during hybridization reactions. The system can be recommended for a rapid and reliable analysis in medical and veterinary practice.",

author = "Kulakov, {I. K.} and Gorelov, {V. N.} and Motin, {V. L.} and Brukhanskiǐ, {G. V.} and Skavronskaia, {A. G.}",

year = "1992",

month = jul,

language = "Russian",

pages = "23--27",

journal = "Molekuliarnaia genetika, mikrobiologiia i virusologiia",

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AU - Kulakov, I. K.

AU - Gorelov, V. N.

AU - Motin, V. L.

AU - Brukhanskiǐ, G. V.

AU - Skavronskaia, A. G.

PY - 1992/7

Y1 - 1992/7

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AB - A non-isotopic amplification system was used to identify and indicate Brucella. The terminal sequences of a protein gene fragment in Brucella outer membrane were identified and direct and reverse primers were chosen for a polymerase chain reaction. (PCR). PCR amplifies a specific DNA fragment, 700 kb in size, only in representatives of the Brucella genus. A probe was design, which is the central part of the amplified DNA fragment, 550 kb in size. Single Brucella cells were detectable with an unlabelled probe in the analyzed samples during hybridization reactions. The system can be recommended for a rapid and reliable analysis in medical and veterinary practice.

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Vysokochuvstvitel'naia neizotopnaia sistema gibridizatsii DNK s primeneniem amplifikatsii (PTsR) dlia identifikatsii i indikatsii brutsell.

Abstract

ASJC Scopus subject areas

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