A genetically encoded photocaged Nε-methyl-l-lysine

Yane Shih Wang; Bo Wu; Zhiyong Wang; Ying Huang; Wei Wan; William K. Russell; Pei Jing Pai; Yin N. Moe; David H. Russell; Wenshe R. Liu

doi:10.1039/c002155e

A genetically encoded photocaged N^ε-methyl-l-lysine

Yane Shih Wang, Bo Wu, Zhiyong Wang, Ying Huang, Wei Wan, William K. Russell, Pei Jing Pai, Yin N. Moe, David H. Russell, Wenshe R. Liu

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

A photocaged N^ε-methyl-l-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N^ε-methyl-l-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

Original language	English (US)
Pages (from-to)	1557-1560
Number of pages	4
Journal	Molecular BioSystems
Volume	6
Issue number	9
DOIs	https://doi.org/10.1039/c002155e
State	Published - Sep 2010
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Molecular Biology

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10.1039/c002155e

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abstract = "A photocaged Nε-methyl-l-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover Nε-methyl-l-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.",

author = "Wang, {Yane Shih} and Bo Wu and Zhiyong Wang and Ying Huang and Wei Wan and Russell, {William K.} and Pai, {Pei Jing} and Moe, {Yin N.} and Russell, {David H.} and Liu, {Wenshe R.}",

year = "2010",

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language = "English (US)",

volume = "6",

pages = "1557--1560",

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T1 - A genetically encoded photocaged Nε-methyl-l-lysine

AU - Wang, Yane Shih

AU - Wu, Bo

AU - Wang, Zhiyong

AU - Huang, Ying

AU - Wan, Wei

AU - Russell, William K.

AU - Pai, Pei Jing

AU - Moe, Yin N.

AU - Russell, David H.

AU - Liu, Wenshe R.

PY - 2010/9

Y1 - 2010/9

N2 - A photocaged Nε-methyl-l-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover Nε-methyl-l-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

AB - A photocaged Nε-methyl-l-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover Nε-methyl-l-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

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DO - 10.1039/c002155e

M3 - Article

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JO - Molecular BioSystems

JF - Molecular BioSystems

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A genetically encoded photocaged N^ε-methyl-l-lysine

Abstract

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