A genetically encoded photocaged Nε-methyl-l-lysine

Yane Shih Wang, Bo Wu, Zhiyong Wang, Ying Huang, Wei Wan, William K. Russell, Pei Jing Pai, Yin N. Moe, David H. Russell, Wenshe R. Liu

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


A photocaged Nε-methyl-l-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover Nε-methyl-l-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

Original languageEnglish (US)
Pages (from-to)1557-1560
Number of pages4
JournalMolecular BioSystems
Issue number9
StatePublished - Sep 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology


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