A facile method to synthesize histones with posttranslational modification mimics

Zhiyong U. Wang; Yane Shih Wang; Pei Jing Pai; William K. Russell; David H. Russell; Wenshe R. Liu

doi:10.1021/bi300535a

A facile method to synthesize histones with posttranslational modification mimics

Zhiyong U. Wang, Yane Shih Wang, Pei Jing Pai, William K. Russell, David H. Russell, Wenshe R. Liu

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Using an evolved pyrrolysyl-tRNA synthetase-tRNA^Pyl pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

Original language	English (US)
Pages (from-to)	5232-5234
Number of pages	3
Journal	Biochemistry
Volume	51
Issue number	26
DOIs	https://doi.org/10.1021/bi300535a
State	Published - Jul 3 2012
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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10.1021/bi300535a

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abstract = "Using an evolved pyrrolysyl-tRNA synthetase-tRNAPyl pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.",

author = "Wang, {Zhiyong U.} and Wang, {Yane Shih} and Pai, {Pei Jing} and Russell, {William K.} and Russell, {David H.} and Liu, {Wenshe R.}",

year = "2012",

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T1 - A facile method to synthesize histones with posttranslational modification mimics

AU - Wang, Zhiyong U.

AU - Wang, Yane Shih

AU - Pai, Pei Jing

AU - Russell, William K.

AU - Russell, David H.

AU - Liu, Wenshe R.

PY - 2012/7/3

Y1 - 2012/7/3

N2 - Using an evolved pyrrolysyl-tRNA synthetase-tRNAPyl pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

AB - Using an evolved pyrrolysyl-tRNA synthetase-tRNAPyl pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

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U2 - 10.1021/bi300535a

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M3 - Article

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JO - Biochemistry

JF - Biochemistry

IS - 26

ER -

A facile method to synthesize histones with posttranslational modification mimics

Abstract

ASJC Scopus subject areas

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