A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Ying Huang; William K. Russell; Wei Wan; Pei Jing Pai; David H. Russell; Wenshe Liu

doi:10.1039/b920120c

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Ying Huang, William K. Russell, Wei Wan, Pei Jing Pai, David H. Russell, Wenshe Liu

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N^ε-acetyl- l-lysines into one GFP_UV protein in E. coli.

Original language	English (US)
Pages (from-to)	683-686
Number of pages	4
Journal	Molecular BioSystems
Volume	6
Issue number	4
DOIs	https://doi.org/10.1039/b920120c
State	Published - 2010
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Molecular Biology

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10.1039/b920120c

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title = "A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli",

abstract = "By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl- l-lysines into one GFPUV protein in E. coli.",

author = "Ying Huang and Russell, {William K.} and Wei Wan and Pai, {Pei Jing} and Russell, {David H.} and Wenshe Liu",

year = "2010",

doi = "10.1039/b920120c",

language = "English (US)",

volume = "6",

pages = "683--686",

journal = "Molecular BioSystems",

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publisher = "Royal Society of Chemistry",

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T1 - A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

AU - Huang, Ying

AU - Russell, William K.

AU - Wan, Wei

AU - Pai, Pei Jing

AU - Russell, David H.

AU - Liu, Wenshe

PY - 2010

Y1 - 2010

N2 - By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl- l-lysines into one GFPUV protein in E. coli.

AB - By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl- l-lysines into one GFPUV protein in E. coli.

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DO - 10.1039/b920120c

M3 - Article

C2 - 20237646

AN - SCOPUS:77949757079

SN - 1742-206X

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EP - 686

JO - Molecular BioSystems

JF - Molecular BioSystems

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ER -

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Abstract

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