A comparison of the 26 S mRNAs and structural proteins of an equine virulent Venezuelan encephalitis virus and its vaccine derivative

James O. Mecham; Dennis W. Trent

doi:10.1016/0042-6822(82)90165-9

A comparison of the 26 S mRNAs and structural proteins of an equine virulent Venezuelan encephalitis virus and its vaccine derivative

James O. Mecham, Dennis W. Trent

Research output: Contribution to journal › Article › peer-review

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Abstract

Fingerprints of the oligonucleotides generated by RNase T₁ digestion of the equine virulent Venezuelan equine encephalitis virus, Trinidad donkey (TRD), intracellular 26 S RNA were similar to those of its vaccine derivative, TC-83 virus. Three oligonucleotides present in the fingerprint of TRD virus 26 S RNA were missing from that of the TC-83 26 S RNA which had three new oligonucleotides. To determine if these genetic changes are expressed in the three structural proteins of the two VEE viruses, we compared the individual proteins from each virus by peptide mapping the trypsin fragments by high-performance liquid chromatography. The nucleocapsid proteins and E, envelope glycoprotein from both viruses produced identical peptide maps; however, analysis of the peptide fragments of the E₂ envelope glycoproteins revealed four different peptide fragments between the two viruses.

Original language	English (US)
Pages (from-to)	251-261
Number of pages	11
Journal	Virology
Volume	121
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(82)90165-9
State	Published - Sep 1982
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(82)90165-9

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title = "A comparison of the 26 S mRNAs and structural proteins of an equine virulent Venezuelan encephalitis virus and its vaccine derivative",

abstract = "Fingerprints of the oligonucleotides generated by RNase T1 digestion of the equine virulent Venezuelan equine encephalitis virus, Trinidad donkey (TRD), intracellular 26 S RNA were similar to those of its vaccine derivative, TC-83 virus. Three oligonucleotides present in the fingerprint of TRD virus 26 S RNA were missing from that of the TC-83 26 S RNA which had three new oligonucleotides. To determine if these genetic changes are expressed in the three structural proteins of the two VEE viruses, we compared the individual proteins from each virus by peptide mapping the trypsin fragments by high-performance liquid chromatography. The nucleocapsid proteins and E, envelope glycoprotein from both viruses produced identical peptide maps; however, analysis of the peptide fragments of the E2 envelope glycoproteins revealed four different peptide fragments between the two viruses.",

author = "Mecham, {James O.} and Trent, {Dennis W.}",

note = "Funding Information: J.O.M. was partially supported by the U. S. Department of Agriculture, Science and Education Administration, Agricultural Research, Arthropod-Borne Animal Disease Research Laboratory, Denver, Colorado 80225.",

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T1 - A comparison of the 26 S mRNAs and structural proteins of an equine virulent Venezuelan encephalitis virus and its vaccine derivative

AU - Mecham, James O.

AU - Trent, Dennis W.

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Y1 - 1982/9

N2 - Fingerprints of the oligonucleotides generated by RNase T1 digestion of the equine virulent Venezuelan equine encephalitis virus, Trinidad donkey (TRD), intracellular 26 S RNA were similar to those of its vaccine derivative, TC-83 virus. Three oligonucleotides present in the fingerprint of TRD virus 26 S RNA were missing from that of the TC-83 26 S RNA which had three new oligonucleotides. To determine if these genetic changes are expressed in the three structural proteins of the two VEE viruses, we compared the individual proteins from each virus by peptide mapping the trypsin fragments by high-performance liquid chromatography. The nucleocapsid proteins and E, envelope glycoprotein from both viruses produced identical peptide maps; however, analysis of the peptide fragments of the E2 envelope glycoproteins revealed four different peptide fragments between the two viruses.

AB - Fingerprints of the oligonucleotides generated by RNase T1 digestion of the equine virulent Venezuelan equine encephalitis virus, Trinidad donkey (TRD), intracellular 26 S RNA were similar to those of its vaccine derivative, TC-83 virus. Three oligonucleotides present in the fingerprint of TRD virus 26 S RNA were missing from that of the TC-83 26 S RNA which had three new oligonucleotides. To determine if these genetic changes are expressed in the three structural proteins of the two VEE viruses, we compared the individual proteins from each virus by peptide mapping the trypsin fragments by high-performance liquid chromatography. The nucleocapsid proteins and E, envelope glycoprotein from both viruses produced identical peptide maps; however, analysis of the peptide fragments of the E2 envelope glycoproteins revealed four different peptide fragments between the two viruses.

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A comparison of the 26 S mRNAs and structural proteins of an equine virulent Venezuelan encephalitis virus and its vaccine derivative

Abstract

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