TY - JOUR
T1 - A change in the stoichiometry of assembly of bovine lens α-crystallin subunits in relation to cellular differentiation
AU - Delcour, Jean
AU - Papaconstantinou, John
N1 - Funding Information:
Energy Commission under contract
PY - 1974/3/15
Y1 - 1974/3/15
N2 - Incorporation of [3H] leucine into oligomeric α-crystallin via individual subunits has been measured in epithelial cells and cortex fiber cells from adult bovine lenses in vitro. Our data show that the ratio of [3H] leucine incorporation via subunits αB2 and αA2 is shifted from a value of about 1:2 in epithelial cells to a value of about 1:3 in fiber cells. Thus, in this system, cellular differentiation is accompanied by a change in the stoichiometry of assembly of individual subunits to form the oligomeric α-crystallin molecule. These results indicate possible changes in the rates of synthesis of individual α-crystallin subunits.
AB - Incorporation of [3H] leucine into oligomeric α-crystallin via individual subunits has been measured in epithelial cells and cortex fiber cells from adult bovine lenses in vitro. Our data show that the ratio of [3H] leucine incorporation via subunits αB2 and αA2 is shifted from a value of about 1:2 in epithelial cells to a value of about 1:3 in fiber cells. Thus, in this system, cellular differentiation is accompanied by a change in the stoichiometry of assembly of individual subunits to form the oligomeric α-crystallin molecule. These results indicate possible changes in the rates of synthesis of individual α-crystallin subunits.
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U2 - 10.1016/S0006-291X(74)80367-0
DO - 10.1016/S0006-291X(74)80367-0
M3 - Article
C2 - 4828184
AN - SCOPUS:0015978270
SN - 0006-291X
VL - 57
SP - 134
EP - 141
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -